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HPLC / GC Training.

We offer training courses in both HPLC & GC to suit anyone new to HPLC or has little HPLC experience.

Refreshments and lunch provided.

Participants receive detailed notes and a certificate upon completion.

An introduction to practical HPLC

Course Contents

Day 1 - non practical session

  • The basic theory of HPLC
  • HPLC system components
    • Solvents
    • Pumps - isocratic / gradient
    • Injection - manual injection valves / autoinjectors
    • Columns - care of columns / performance of column
    • Detectors - choosing the right detector
    • Data systems - integrators / software
  • Q&A session

Day 2 - practical session

  • System Setup
  • Practical session
    • Eluent preparation
    • Sample preparation
    • Sample injection
    • Data analysis
  • HPLC 1st line maintenance which covers the inspection & removal of
    • Piston seals
    • Pistons
    • Checkvalves
    • Rotor seal
    • Detector lamp
  • Q&A session

Upon completion of the course you should be in a position to

  • Choose your HPLC system
  • Prepare your HPLC system including the eluent
  • Prepare your sample & calibration samples
  • Inject your samples
  • Calibrate your HPLC system
  • Determine the concentration of the analyte in your sample

 

Basic Gas Chromatography

Our GC course covers the following topics.

  • Basic principles of GC
  • System components
    • Carrier gas selection
    • Chromatographic columns
    • Flow control
  • Sample introduction
    • Manual injection
    • Auto injection
    • Split/splitless injection
    • Septum purge
  • Temperature programming
  • Detectors
    • FID
    • TCD
    • ECD
  • Practical Session
    • Manual sample injection
    • Optimizing a temperature program
    • Adjusting split ratio & septum purge

 

GC 1st line maintenance covers the inspection & removal of

  • Septa
  • Liner
  • Columns
  • Detector collector & jet