HPLC / GC Training.
We offer training courses in both HPLC & GC to suit anyone new to HPLC or has little HPLC experience.
Refreshments and lunch provided.
Participants receive detailed notes and a certificate upon completion.
An introduction to practical HPLC
Course Contents
Day 1 - non practical session
- The basic theory of HPLC
- HPLC system components
- Solvents
- Pumps - isocratic / gradient
- Injection - manual injection valves / autoinjectors
- Columns - care of columns / performance of column
- Detectors - choosing the right detector
- Data systems - integrators / software
- Q&A session
Day 2 - practical session
- System Setup
- Practical session
- Eluent preparation
- Sample preparation
- Sample injection
- Data analysis
- HPLC 1st line maintenance which covers the inspection & removal of
- Piston seals
- Pistons
- Checkvalves
- Rotor seal
- Detector lamp
- Q&A session
Upon completion of the course you should be in a position to
- Choose your HPLC system
- Prepare your HPLC system including the eluent
- Prepare your sample & calibration samples
- Inject your samples
- Calibrate your HPLC system
- Determine the concentration of the analyte in your sample
Basic Gas Chromatography
Our GC course covers the following topics.
- Basic principles of GC
- System components
- Carrier gas selection
- Chromatographic columns
- Flow control
- Sample introduction
- Manual injection
- Auto injection
- Split/splitless injection
- Septum purge
- Temperature programming
- Detectors
- FID
- TCD
- ECD
- Practical Session
- Manual sample injection
- Optimizing a temperature program
- Adjusting split ratio & septum purge
GC 1st line maintenance covers the inspection & removal of
- Septa
- Liner
- Columns
- Detector collector & jet





